ganQ [2019-09-25 09:30:21]

galactotriose ABC transporter (permease)

Locus: BSU_34140

Isoelectric point: 9.76

Molecular weight: 31.15 kDa

Protein length: 283 aa

Gene length: 852 bp

Function: uptake of galactotriose

Product: galactotriose ABC transporter (permease)

Essential: no

Synonyms: yvfM

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of galactan
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,505,102 3,505,953

Phenotypes of a mutant

• a ganS-ganP-ganQ-ganA-ganB mutant is impaired in the utilization of galactan PubMed

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)

Paralogous protein(s)

• MdxG

Structure

• 2R6G (from E. coli, 34% identity) PubMed

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: ganS-ganP-ganQ-ganA-ganB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • GanR: repression, PubMed, in GanR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • induced by galactan (binding of galactobiose to GanR) PubMed
  • repressed by glucose (CcpA) PubMed

Biological materials

Mutant

• MGNA-A486 (yvfM::erm), available at the NBRP B. subtilis, Japan
• BKE34140 (ganQ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GACTTTCATATCTGCCAGCA, downstream forward: _UP4_GGCTAAGAGAACAAGGAGGA
• BKK34140 (ganQ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GACTTTCATATCTGCCAGCA, downstream forward: _UP4_GGCTAAGAGAACAAGGAGGA

References

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